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Gibco™ CTS™ Synth-a-Freeze™ Medium

CTS (Cell Therapy Systems) Synth-a-Freeze medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing a variety of mammalian cell types including stem cells.

Supplier:  Gibco™ A1371301


Catalog No. A1371301

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CTS™ (Cell Therapy Systems) Synth-a-Freeze™ medium is a chemically defined, protein-free liquid cryopreservation medium intended for freezing and storing a variety of mammalian cell types including stem cells. Synth-a-Freeze™ CTS™ media is optimally formulated without antibiotics, antimycotics, hormones, growth factors, serum, or protein and buffered with HEPES and sodium bicarbonate.

• Use for cryopreserving a variety of primary and stem cells
• Save time and effort with 4 degree stable storage of thawed media and compatibility with a variety freezing protocols
• Be ready for clinical research with this cGmP manufactured, chemically defined, protein-free formula

Cryopreserve a Variety of Cell Types
Cell Therapy Systems Synth-a-Freeze™ CTS™ media performs comparable to our standard, serum-containing cryopreservation medium for cryopreserving a variety of cell types including human Embryonic Stem Cells, Neural Stem Cells (Ref 1), Mesenchymal Stem Cells and Keratinocytes (Ref 2). We do not recommend using Synth-a-Freeze™ media for cryopreservation of human epidermal melanocytes.

Simple Incorporation into Your Current Freezing Protocol
Synth-a-Freeze™ CTS™ media can be used with any standard freezing protocol. Just add thawed Cell Therapy Systems Synth-a-Freeze™ CTS™ media to detached and pelleted cells, aliquot, and freeze. For optimal results, the use of a controlled-rate freezer is recommended.

Simplify Your Transition into Clinical Research
The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ Cell Therapy Systems (CTS™) products deliver:
• Reduced burden in qualifying reagents
• Included appropriate certificates of analyses and origin
• Convenient access to our Drug Master File (DMF)

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.

CAUTION: Not intended for direct administration into humans or animals

• S. Shin and M. Vemuri. Culture and Differentiation of Human Neural Stem Cells. L.C. Doering (ed). Protocols for Neural Cell Culture. Springer Protocol Handbooks.
• S. Lemaire, K. Kosowska-Shick, P. Appelbaum, G. Verween, P. Tulkens, and F. Van Bambeke. Cellular Pharmacodynamics of the Novel Biaryloxazolidinone Radezolid: Studies with Infected Phagocytic and Nonphagocytic cells, Using Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, and Legionella pneumophila. Antimicrob Agents Chemother 54(6) 2010: 2549-2559.



Store at –20°C.
Animal Origin-free
Cryopreservation Medium
DMSO (10%)
Cell and Gene Therapy Research, Development, and Manufacturing
50 mL
Various (including types of human Embryonic Stem Cells, Neural Stem Cells, Mesenchymal Stem Cells, and Keratinocytes)
ISO 13485, MDSAP, FDA-registered, 21 CFR 820
Dry Ice
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ShelfLife : 18 months from date of manufacture



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For Research Use Only. Not for use in diagnostic procedures.