Corning™ HEPES, Liquid

An alternative to use of bicarbonate for buffering

Supplier:  Corning™ 25060CI

Catalog No. MT25060CI

Description

Technical Advantage:  Product manufactured under industry-leading standards for low endotoxin content and superior results.
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2.  Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer.  Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.  The recommended working concentration is 10-25 mM.
-  Complete Certificate of Analysis available for each production lot, along with full formulation information

Corning media certificates are available on our Resource Library.

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Specifications

• Content And Storage: 15° to 30°C
• Product Type: HEPES Buffer
• pH: 7.2 to 7.6

• Form: Liquid
• Sterility: Sterile-filtered
• Quantity: 6 x 100 mL