Collagen I Monoclonal Antibody (COL-1), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA126771

Catalog No. MA126771

Description

The antibody is reactive with the native (helical) form of collagen type I in ELISA and dot blot. The product is not reactive when tested on thermally denatured molecules. In immunohistochemical staining of acetone-fixed and unfixed frozen sections, a strong staining of connective tissue fibers is seen. Using the dot blot and ELISA techniques, the antibody shows no cross-reactivity with collagen types, II, III, IV, V, VI, VII, IX, X and XI. The epitope recognized by the antibody is sensitive to routine formalin fixation and paraffin embedding.|Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

Specifications

• Antigen: Collagen I
• Classification: Monoclonal
• Concentration: 7.4 mg/mL
• Formulation: ascites with 15mM sodium azide
• Gene Accession No.: P02452, P02453, P02454, P02456, P02465, P02466, P08123, P11087, Q01149, Q28668
• Gene Symbols: COL1A1, COL1A2
• Immunogen: Bovine skin collagen type I
• Regulatory Status: RUO
• Gene ID (Entrez): 100008997, 100347598, 100626716, 100738123, 1277, 1278, 12842, 12843, 282187, 282188, 29393, 84352
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Dot Blot, ELISA, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: COL-1
• Conjugate: Unconjugated
• Gene: COL1A2
• Gene Alias: AA960264; AI325291; alpha 1 type I collagen; alpha 2 type I collagen; alpha 2 type I procollagen; alpha 2(I) collagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha1 chain of type I collagen; alpha-1 collagen (I); alpha-1 type 1 collagen; alpha-1 type I collagen; alpha-1 type-1 collagen; alpha1(I) procollagen; alpha2 chain of type I collagen; alpha-2 type I collagen; alpha-2(1) collagen; alpha2(I) collagen; COL1; Col1a1; Col1a-1; COL1A1; matrix protein; Col1a2; Col1a-2; COLA; Cola1; Cola-1; Cola2; Cola-2; COLIA1; collagen; collagen 1 alpha 2 chain; collagen a2; collagen alpha 1 chain type I; collagen alpha 2(I) chain precursor; collagen alpha-1 chain; collagen alpha-1 chain precursor; collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen alpha-2; collagen alpha-2 chain precursor; collagen alpha-2(I) chain; collagen COL1A2; collagen I alpha-2 polypeptide; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tend
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Deer, Human, Mouse, Porcine, Rabbit, Rat
• Product Type: Antibody
• Isotype: IgG1