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CHOP Monoclonal Antibody (9C8), Invitrogen™
Mouse Monoclonal Antibody
Supplier: Thermo Scientific MA1250
Description
MA1-250 detects CHOP protein in human and mouse samples. MA1-250 has successfully been used in Western blot, immunoprecipitation, immunocytochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ∽31 kDa protein representing CHOP from primary human fibroblast extract. The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP. This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.Specifications
CHOP | |
Monoclonal | |
1 mg/mL | |
PBS with 1mg/mL BSA and 0.05% sodium azide | |
P35638, P35639 | |
DDIT3 | |
A bacterially expressed, mouse CHOP fusion protein. | |
100 μg | |
Primary | |
Human, Mouse | |
Antibody | |
IgG2b |
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot | |
9C8 | |
Unconjugated | |
DDIT3 | |
C/EBP homologous protein; C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CEBPZ; CHOP; CHOP10; CHOP-10; Ddit3; DDIT-3; ddit3.L; DNA damage inducible transcript 3; DNA damage inducible transcript 3 L homeolog; DNA damage-inducible transcript 3 protein; DNA-damage inducible transcript 3; DNA-damage-inducible transcript 3; GADD153; growth arrest and DNA damage-inducible; growth arrest and DNA damage-inducible protein GADD153; Growth arrest and DNA-damage-inducible protein GADD153; MGC4154; transcription factor GADD153; Xchop; XELAEV_18013440mg | |
Mouse | |
Protein A | |
RUO | |
13198, 1649 | |
-20° C, Avoid Freeze/Thaw Cycles | |
Liquid |
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