MilliporeSigma™ Calbiochem™ TMB, Soluble Substrates for AP and HRP

Peroxidase substrate that produces blue soluble end product that can be read at 370nm or 650nm. Calbiochem™ TMB, Soluble Substrates for AP and HRP use of stop solution enhances sensitivity two- to four-fold and results in yellow solution that can be read at 450nm.

Supplier:  MilliporeSigma™ 613544100ML

Catalog No. 61-354-4100ML

Description

• CAS number: 54827-17-7
• Form: Liquid, clear to very light blue
• Preservative: None
• Special instructions: Protect from exposure to direct sunlight. Discard if the solution turns blue or turbid. The reagent is stable at room temperature, but we recommend storage in the refrigerator for longer shelf life. Warm to assay temperature before use.

Specifications

• Product Type: TMB, Soluble
• For Use With (Application): ELISA
• Storage Requirements: Protect from light. Ok to freeze.
• Form: Liquid

• Formulation: Single component system containing 1.46mM TMB, 2.21mM H_₂O_₂ in a proprietary solvent
• pH: 3.1 ± 0.5
• Volume (Metric): 100mL