Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.

Supplier:  Thermo Scientific™ EP0691

Catalog No. FEREP0691

Description

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E. coli cells with a cloned polA gene from Bacillus smithii, Bsm DNA Polymerase, Large Fragment, features strong strand displacement activity and is active in a wide range of temperatures (30–63°C), with an optimum of activity at 60°C. With high functional similarity to Bst DNA Polymerase, Large Fragment, this enzyme can serve as alternative in most applications including loop-mediated isothermal amplification (LAMP).

Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E. coli cells with a cloned polA gene from Bacillus smithii, Bsm DNA Polymerase, Large Fragment, features strong strand displacement activity and is active in a wide range of temperatures (30–63°C), with an optimum of activity at 60°C. With high functional similarity to Bst DNA Polymerase, Large Fragment, this enzyme can serve as an alternative in most applications, including loop-mediated isothermal amplification (LAMP).

Applications
Bsm DNA Polymerase, Large Fragment, may be used in a wide variety of applications including:
• Loop-mediated isothermal amplification (LAMP)
• Whole genome amplification (WGA)
• Ramification amplification (RAM)
• Random-primed DNA labeling
• Labeling by fill-in 5'-overhangs of dsDNA

Specifications

• Product Type: Stand-alone enzyme
• Polymerase: Bsm DNA Polymerase
• Content And Storage:

  • Bsm DNA Polymerase, Large Fragment (200 μL at 8 U/μL)
  • 10X Bsm Buffer (1.25 mL)
  
  Store at –15 to –25°C.
  
  

• Shipping Condition: Dry Ice
• Reaction Time: 30 to 60 minutes

• For Use With (Application): LAMP, RT-LAMP, WGA, RAM
• Quantity: 1600 U
• Optimal Reaction Temperature: 60°C
• Concentration: 8 U/μL

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.