Invitrogen™ BLC/CXCL13 Mouse ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ EMCXCL13

Catalog No. EMCXCL13

Description

The Mouse BLC/CXCL13 ELISA quantitates Ms BLC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BLC. Principle of the method The Mouse BLC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles.

Specifications

• Accession Number: O55038
• Assay Sensitivity: 2 pg/mL
• Description: BLC (CXCL13) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BLC (CXCL13) in Samples Using a Microplate Reader
• Assay: Solid-phase sandwich ELISA
• Detection Method: Colorimetric
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 Assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 55985
• Host Species: Mouse
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 5 μL; Serum, 5 μL; Supernatant, 100 μL
• Synonym: 4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13
• Test Time: 1 hr. 20 min.

• Assay Range: 1.37-1000 pg/mL
• Calibration Range: 2pg/mL to 2000pg/mL
• Detectable Analytes: BLC (CXCL13), mouse BLC
• Conjugate: Biotin
• Immunoassay Kit Format: Sandwich ELISA Kit
• Protein Family: Chemokine & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Cxcl13
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse BLC precoated 96-well strip plate, lyophilized recombinant mouse BLC standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentrated b
• Quantity: 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.