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Phosflow Lyse/Fix Buffer 5X, BD

For the lysing and fixing of whole blood for use with intracellular flow cytometry

Supplier:  BD Biosciences 558049

Catalog No. BDB558049

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  • Can be used for lysing red blood cells and for fixing leukocytes in one step, allowing investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that the in vivo phosphorylation state of the cell can be examined
  • Has been reported to preserve the light scattering properties of the cell
  • May be used on human and mouse whole blood
  • Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
  • In many cases, phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however, not all cell surface markers, however, are compatible with the BD Phosflow Lyse/Fix Buffer



Lyse/Fix Buffer
Room Temperature
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