BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit with BD GolgiStop™
Provides GolgiStop protein transport inhibitor containing monensin
Supplier: BD 554715
- Fixation/Permeabilization solution (125mL)
- BD Perm/Wash™ Buffer, 10x concentrate containing Fetal Bovine Serum (FBS) and saponin (100mL)
- Protein transport inhibitor BD GolgiStop™ (containing Monensin)
The ex vivo addition of GolgiStop to in vitro- or in vivo -stimulated cells blocks their intracellular transport processes. This results in the accumulation of most cytokine proteins in the Golgi complex and thereby enhances cytokine staining signals.
- GolgiStop reagent is provided for treating up to 1L of cell culture at a cell density of up to 2 × 106 cells/mL
|125 mL fixation/permeabilization solution, perm/Wash™ buffer, fetal bovine serum (FBS) 10X and 100 mL saponin, protein transport inhibitor BD GolgiStop™|
|Fixation/Permeabilization Solution Kit|
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