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BD BrdU In-Situ Detection Kit

Determines proliferation rates and cell cycle kinetics in normal and cancerous tissues

Supplier:  BD 550803

Catalog No. BDB550803


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Alert:

Highly toxic, avoid contact with skin, eyes, and mucous membranes


Includes: Fixation Buffer, Diluent Buffer, BD™ Retrievagen A Antigen Retrieval Solution, biotinylated anti-BrdU antibody, ready-to-use Streptavidin-Horseradish peroxidase (SAv-HRP) Solution, DAB Buffer, DAB Chromogen, and Control Slides

Requires : BrdU for in vitro or in vivo labeling, H2O2 (30%) solution for blocking endogenous peroxidase, phosphate buffer saline (PBS)

Description

Description

Cellular incorporation of BrdU during S phase can be readily detected by anti-BrdU-specific antibodies following membrane permeabilization. Subsequent quantitation of cells positive for BrdU is possible using routine methods for cell analysis

  • Measures labeling indices of different cell populations
  • Improved specific staining with minimal background
  • Designed antigen retrieval solution unmask antigenic sites and preserve tissue morphology
  • Enables study of proliferation state of phenotypically defined cells within the micro-environment of tissues
  • Reaction: 50 slides

Flow Cytometry, Immunohistochemistry

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Specifications

Specifications

1 Kit
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Research use only