Cytiva bacteria genomicPrep Mini Spin Kit

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria

Catalog Number	Mfr. No.	Includes	No. of Reactions
45-001-494	Cytiva 28904259	30mg Proteinase K, 3mL Lysis Buffer type 2, 0.6mL Lysis Buffer type 3, 60mL Lysis Buffer type 4, 6mL Wash Buffer type 6, 12mL Elution Buffer type 5, 50 Illustra™ bacteria mini columns, 50 collection tubes	250 Preps

Description

The illustra Bacteria genomicPrep Mini Spin Kit can be used with a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis etc. The kit produces highly pure and intact genomic DNA with OD_260/280 values of 1.75-1.85. Use different elution volumes to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RT-PCR, and long PCR.

The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.

• Total DNA extraction time under one hour with reduced lysis time
• Can be used to purify 4 to 8μg of genomic DNA from 2 × 10⁹ bacteria cells with input amounts ranging from 1 × 10⁹ to 4 × 10⁹ bacteria cells
• Spin columns prepacked with a silica membrane—provide excellent reproducibility and consistency
• Validated in several downstream applications including RT PCR, long PCR, and restriction digest
• Color-coded caps for ease of use

Specifications

• Description: illustra bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from Gram-negative (G-) and Gram-positive (G+) bacteria.
• Product Type: illustra bacteria genomicPrep Mini Spin Kit
• Sample Volume: 2 x 10⁹ cells [A₆₀₀ = 2.0]

• For Use With (Application): For the rapid extraction and purification of high-quality genomic DNA from gram-positive and gram-negative bacteriaPurified gDNA is suitable for molecular biology applications including cloning, restriction enzyme digestion, PCR and genotyping
• Purity: 1.7
• Test Time: 40 min [Gram-negaitive Bacteria], 55 min [Gram-positive Bacteria]