Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Bac-to-Bac™ C-His TOPO™ Cloning Kit

Bac-to-Bac™ C-His TOPO™ Cloning Kit

Supplier:  Invitrogen™ A11098

Encompass_Preferred

Catalog No. A11098


Add to Cart

Description

Description

Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells. New: pFastBacTOPO™ vectors!


Reliable and fast protein expression
The Bac-to-Bac™ Baculovirus Expression System is faster and easier than traditional baculovirus expression, and it maintains high levels of protein expression. Bac-to-Bac™ relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells. The Bac-to-Bac™ Baculvirus Expression System is highly regarded in academic Literature. Approximately 100 citations every year since 2000!

Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. This requires a time-consuming plaque assay. Bac-to-Bac™'s pFastBac™ vector, however, recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid. Transfect the bacmid into insect cells for fast production of a high titer of pure recombinant baculovirus particles in the very first transfection. You'll save weeks of precious time. Collect a pure P2 baculovirus stock on Day 10 without the necessity of tedious plaques assay (See Figure 1)

High expression, easy screening
pFastBac™ uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell lines such as Sf9, Sf21, and High Five™ cells.

New additions:
Bac-to-Bac™'s pFastBac vectors are now available as pFastBacTOPO™ vectors too. pFastBacTOPO™ vectors are Blunt™ TOPO™ vectors and are supplied with Mach1™-T1R E. coli for easy cloning and pFastBac⁄GOI propagation. The new vectors enable you to:

  • Amplify your gene of interest (GOI) with a PCR enzyme of highest fidelity such as Accuprime™ Pfx SuperMix.

  • TOPO™ clone the blunt-end PCR product into the new vector in only 5 minutes!

  • Visualize colonies 8 hours after plating on ampicillin selective plates because Mach1™-T1R E. coli cells have a faster doubling time compared to other standard cloning strains.

  • With the Bac-to-Bac™ C-His TOPO™ cloning or expression kit, you can produce a C-terminal His-fusion protein with a TEV cleavage site to purify with nickel-chelating resins (Invitrogen’s ProBond™ Purification System) and generate a native protein with the aid of Invitrogen’s popular AcTEV™ Protease. This is the Invitrogen’s only vector that contains a C-terminal TEV cleavage site!

    The new vectors combine the TOPO™ cloning technology with the highly regarded Bac-to-Bac™ baculovirus expression features to enable easy cloning and high-level protein expression.
    The pFastBacTOPO™ vectors are offered as cloning and expression kits.
    TRUSTED_SUSTAINABILITY
  • Specifications

    Specifications

    Ampicillin (AmpR), Gentamicin (GmR)
    Insect Cell
    Kit content for Bac-to-Bac™ C-His TOPO™ cloning kit (For details see the manual)
    • Vector kit for 20 topo cloning reactions:
    o pFastBac⁄CT-TOPO™ Vector containing the C-terminal TEV cleavage sit and His-Tag.
    o pFastBac⁄CT-Gus Control Plasmid (Control expression vector)
    o Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer
    • Kit containing competent cells (20 reactions):
    o One Shot™ Mach1-T1R Chemically Competent E. coli

    Vector kit: store at -20°C
    One Shot™ Mach1-T1R Chemically Competent E. coli: store at -80°C
    Baculovirus
    20 Reactions
    Cloning Kit
    His Tag (6x)
    Ampicillin, Gentamicin
    High Five™, Sf21, Sf9
    Blunt TOPO
    Insect Cell Expression
    Protein Expression, Viral Vaccine Production
    Bac-to-Bac™, TOPO™
    Polyhedrin
    20 Reactions
    pFastBac/CT-TOPO
    Product Suggestions

    Product Suggestions

    SDS
    Documents

    Documents

    Product Certifications
    Promotions

    Promotions

    Provide Content Correction

    The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Cancel Submit

    For Research Use Only. Not for use in diagnostic procedures.