Arginase 1 Monoclonal Antibody (A1exF5), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
46-369-780	eBioscience 46369780	25μg
46-369-782	eBioscience 46369782	100μg

Description

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both, the standard intracellular protocols, and the Foxp3/Transcription Factor Staining Buffer Set. Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of stimulated mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Specifications

• Antigen: Arginase 1
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: AI; A-I; AI type I arginase; AI256583; Arg1; Arg-1; Arginase; arginase 1; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase1; arginase-1; HGNC:663; Liver Arginase; Liver-type arginase; PGIF; similar to arginase, type I; Type 1 Arginase; type I arginase
• Gene Symbols: ARG1
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: A1exF5
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Target Species: Human, Mouse
• Gene Accession No.: P05089, Q61176
• Gene ID (Entrez): 11846, 383
• Immunogen: E.coli-derived Recombinant mouse Arginase 1
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: ARG1