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Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Supplier: Applied Biosystems™ 4403313
Includes: Reagents sufficient for 100 sample preparation reactions at 50μL/ reaction and 400 PCR reactions at 5μL for each reaction.
Description
The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.
- DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min.
- The TaqMan™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min.
- Compatible with both Fast and Standard mode thermal cycling conditions
- Pre-mixed components stored at 2° to 8°C significantly reduce assay setup time
- Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
- Extended PCR cycling for poorly amplified or sample-limited reactions
Order Info
Guaranteed minimum shelf life is 60 days.
Specifications
Lysis Reagents (2° to 8°C): 5 mL each Lysis and DNA Stabilization Solution; Master Mix (15° to 25°C ): 1 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye and ROX™ Dye. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
|
Low | |
AmpliTaq DNA Polymerase | |
SNP Genotyping (Real-Time PCR-Based) | |
SNPs (Unknown or Numerous), SNPs (Known) | |
Sample-to-SNP™, TaqMan™ | |
UP (Ultra Pure) | |
Built-In Hot Start | |
5 mL |
Primer-probe | |
qPCR | |
Fast or Standard | |
7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOne™, Fast Mode, StepOne™, Standard Mode, Veriti Thermal Cycler, StepOne™ Standard Mode, StepOne™ Fast Mode | |
ROX (Pre-mixed) | |
Sample to SNP Kit | |
100 Reactions | |
Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
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For Research Use Only. Not for use in diagnostic procedures.