CD8 Mouse anti-Human, Alexa Fluor 700, Clone: RPA-T8, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 561453

Catalog No. BDB561453

Description

Clone RPA-T8 reacts with the α subunit (32kDa) of the two-chain complex expressed on T-cytoxic/suppressor cell populations. CD8 molecule binds to HLA class I molecules during interaction of CD8 +T cells with antigen-presenting cells or with target cells. RPA-T8 reacts with approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. RPA-T8 and HIT8a (Mfr. No.555337) are not cross-blocking. This clone also reacts with a subset of peripheral blood lymphocytes, but not monocytes nor granulocytes, of baboon and both rhesus and cynomolgus macaque monkeys. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD8
• Classification: Monoclonal
• Conjugate: Alexa Fluor 700
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Human CD8a
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: RPA-T8
• Description: CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Isotype: IgG1 κ