BrdU Mouse, V450, Clone: 3D4, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 560810

Catalog No. BDB560810

Description

Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the cells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics.

The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU.

Specifications

• Antigen: BrdU
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: 5-bromo-2'-deoxyuridine, 5-Bromouracil deoxyriboside, BUdR
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 3D4
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgG1 κ