Invitrogen™ TRIzol™ Reagent

TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.

Catalog Number	Mfr. No.	Quantity
15-596-026	Invitrogen™ 15596026	100 mL
15-596-018	Invitrogen™ 15596018	200 mL

Description

TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

Key features of TRIzol™ Reagent include:
• Permits the isolation of RNA, DNA, and protein from the same sample
• Offers superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria

Reliably purify RNA from multiple sample volumes and sources
TRIzol™ Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 10⁶), as well as with large quantities of tissue (≥1 g) and cells (>10⁷), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol™ Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol™ Reagent is free of protein and DNA contamination.

Formulated for isolation of multiple molecular targets
TRIzol™ Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

Order Info

Shipping Condition: Room temperature

Specifications

• Isolation Technology: Organic Extraction
• Elution Volume: 20 to 600 μL
• Final Product Type: Total RNA, DNA, Protein
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis
• Starting Material Amount: Bacteria: ≤10⁷ cells
  Cells: ≤10⁷
  Plant: ≤10⁷ cells, 100 mg
  Tissue: ≤100 mg
  Yeast: ≤10⁷ cells

• Purification Time: 1 hr.
• Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: DNA: ≤7 μg
  RNA: ≤73 μg
  Protein: varies

For Research Use Only. Not for use in diagnostic procedures.