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Invitrogen™ Ambion™ RNase I, cloned, 100 U/μL
Efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues
Supplier: Invitrogen™ AM2295
Description
RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays.
- Supplied in one tube of 10,000U (100U/μL)
- Endonuclease-free, exonuclease-free, protease-free
- Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity
- Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min. at 37°C
DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting
Order Info
Shipping Conditions: Dry ice
Specifications
| 100 U/μL | |
| Dry Ice | |
| 25,000 U | |
| Ambion™ |
| -20°C | |
| RNase | |
| RNase I |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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