Invitrogen™ RNAqueous™ Total RNA Isolation Kit

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 10⁷ to 10⁸ cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

Supplier:  Invitrogen™ AM1912

Catalog No. AM1912

Description

The RNAqueous Kit is for phenol-free total RNA isolation from 0.1–0.5 g of tissue or from 10⁷ to 10⁸ cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

Not high-throughput compatible (Manual)

Applications:
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues

Specifications

• Content And Storage: • 6 mL Formaldehyde Load Dye; store at -20°C
  • 60 mL Lysis/Binding Solution; 4°C
  • 60 mL Water; 4°C
  • 4 mL Lithium Chloride Precipitation Solution; 4°C
  • 40 mL Wash Solution #1; 4°C
  • 80 mL Wash Solution #2/3 (concentrated); 4°C
  • 50 Filter Cartridges; room temperature
  • 100 Collection Tubes; room temperature
  • 10 mL Elution Solution; room temperature
• Elution Volume: 50–120 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Shipping Condition: Room Temperature

• Isolation Technology: Spin Column (Glass Fiber Filter)
• Sample Type: Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Cells: ≤10⁷
  Tissue: ≤75 mg
  Plant: ≤75 mg
  Yeast: ≤10⁸ cells
  Virus: varies
  Enzymatic Reactions: varies

For Research Use Only. Not for use in diagnostic procedures.