Invitrogen™ MAXIscript™ T7/T3 Transcription Kit

Fast synthesis of RNA probes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations

Includes: T7 Enzyme Mix, T3 Enzyme Mix, 10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, pTRI-b-actin, Nuclease-free Water

Catalog Number	Mfr. No.	Product Type	No. of Reactions
AM1326	Invitrogen™ AM1326	T7/T3 In Vitro Transcription kit	100 Reactions
AM1324	Invitrogen™ AM1324	T7/T3 In Vitro Transcription kit	30 Reactions

Description

The Ambion MAXIscript T3/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 15 reactions of each enzyme. MAXIscript Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar™ Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6μg/20μL reaction) of up to 2kb.

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, Northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality tested DNase/RNase-free reagents
• The MAXIscript In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 ×10⁹ cpm/μg in just 10 minutes

Specifications

• Content And Storage: T7 Enzyme Mix, T3 Enzyme Mix,10X Transcription Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, TURBO DNase, Gel Loading Buffer II, and pTRI-b-actin are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
• Format: Kit
• For Use With (Application): In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
• Product Type: T7/T3 In Vitro Transcription kit
• Shipping Condition: Dry Ice
• Starting Material: PCR Products, Linearized DNA plasmid

• Final Product Type: Synthetic mRNA, mRNA
• Sample Type: mRNA, Synthetic mRNA
• Product Line: Ambion™, MAXIscript™
• Promoter: T3, T7
• Yield: 2-6 μg

For Research Use Only. Not for use in diagnostic procedures.