Invitrogen™ GLOBINclear™ Kit, mouse/rat, for globin mRNA depletion

Employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood

Supplier:  Invitrogen™ AM1981

Catalog No. AM1981

Includes: 20μL Capture Oligo Mix; 600μL Streptavidin Magnetic Beads; 1.5mL 2X Hybridization Buffer; 1.5mL Streptavidin Bead Buffer; 1.75 Nuclease-free Water; 200μL RNA Binding Beads; 5mL RNA Wash Solution Concentrate; 1mL Elution Buffer; 80μL RNA Bead Buffer; 4mL RNA Binding Buffer Concentrate; 100 1.5-ml Non-stick Tubes

Description

The Ambion™ GLOBINclear™-Mouse/Rat Kit employs a novel, non-enzymatic technology that rapidly depletes >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood. The kit includes sufficient reagents for 20 reactions.

• Remove >95% of unwanted globin mRNA from whole blood total RNA
• Detect up to 50% more genes—analyze previously undetected genes
• Achieve greater representation of the genome, low 3'/5' ratios
• Avoid RNase H treatments that can degrade mRNA and alter expression profiles

It is widely known that expression array data generated from whole blood total RNA samples have reduced detection sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a dominant band in the 600–700 base region. This band represents globin mRNA, which is expressed at high levels in red blood cells (RBCs) and reticulocytes (RBC precursors). Up to 70% of the mRNA (by mass) in whole blood total RNA are globin transcripts. Array analysis using total RNA containing a high percentage of globin mRNA has been shown to:

• Decrease present calls
• Decrease call concordance
• Increase signal variation

The GLOBINclear™ kit employs a novel hybridization technology that takes advantage of the strength of biotin/streptavidin binding, the specificity of nucleic acid hybridization, and the convenience of magnetic separations. There are no harsh RNase H enzymatic treatments that may compromise the expression profile by degrading mRNAs other than globin mRNA. The resulting mRNA is a superior template for synthesizing labeled cDNA for array analysis and is ideal for quantitative RT-PCR. The enrichment delivers a significant increase in Percent Present calls, decreased sample-to-sample variability, and low 3'/5' ratios.

Accessory Product:
The kit requires the use of a magnetic stand. The 6 Tube Magnetic Stand (SKU# AM10055) can hold six separate 1.5 mL conical microcentrifuge tubes or 2 mL round-bottomed microtubes with a diameter of 8–10 mm.

Order Info

Shipping Condition: Wet/Dry Ice

Specifications

• Product Type: Mouse/Rat Kit
• Final Product Type: Total RNA (Globin mRNA-Depleted)
• Sample Type: Total RNA (Whole Blood)
• Format: Microtube
• For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction

• Content And Storage: • 20 μl Capture Oligo Mix (-20°C)
  • 600 μl Streptavidin Magnetic Beads (4°C—do not freeze)
  • 1.5 ml 2X Hybridization Buffer (room temperature)
  • 1.5 ml Streptavidin Bead Buffer (room temperature)
  • 1.75 Nuclease-free Water (-20°C, 4°C, or room temperature)
  • 200 μl RNA Binding Beads (4°C—do not freeze)
  • 5 ml RNA Wash Solution Concentrate (4°C or room temperature)
  • 1 ml Elution Buffer (4°C or room temperature)
  • 80 μl RNA Bead Buffer (room temperature)
  • 4 ml RNA Binding Buffer Concentrate (room temperature)
  • 100 1.5-ml Non-stick Tubes (room temperature)
• High-throughput Compatibility: High-throughput Compatible
• Species: Mouse, Rat
• Product Line: Ambion™, GLOBINclear™
• Quantity: 20 Preps

For Research Use Only. Not for use in diagnostic procedures.