Edvotek™ AIDS Kit I: Simulation of HIV-1 Detection
In this experiment, students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.
Supplier: Edvotek™ 271
Includes: Complete instructions, background information, and study questions; biologicals and reagents; three microtiter plates; 50 transfer pipettes; 60 microtest tubes with attached caps; twenty-five 1mL pipettes; two 50mL plastic tubes.
- Distilled or deionized water
- Beakers z 37°C incubation oven
- Automatic micro- pipettes and tips (optional)
DescriptionA model for HIV-1 detection without the use of blood
Simulates the screening for antibodies against human immunodeficiency virus (HIV-1). Enzyme-linked immunoabsorbent assay (ELISA) is currently used for initial clinical screening of the blood supply for HIV-1. Students add simulated HIV antigen to wells in the microtiter plate and follow it with washing and introduction of simulated antibodies of HIV-1 positive or negative samples. A second antibody is added which binds to the first antibody of HIV-1 positive samples and results in a color change.
Note: some kit contents may need to be redeemed by mail.
Kit ships with plasticware and instructions on how to obtain remaining materials directly from the supplier, Edvotek.
|Detects hiv infection indirectly using an elisa test against hiv antibodies in the blood|
|Aids Kit I|
|Instructions, simulated hiv antigens, serum samples, antibodies, buffers, microtiter plates, assorted pipets and microtest tubes|
|10 Lab Groups|
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