BTX™ AgilePulse™ MAX Large Volume Transfection System

AgilePulse™ MAX Large Volume Transfection System provides an advanced electroporation solution for fast, efficient transfection of up to 10 mL of cell suspension.

Supplier:  BTX™ 470200N

Catalog No. BTX470200N

Description

Includes a user-friendly, programmable waveform generator with patented AgilePulse™ technology, BTX™ patent-pending large-volume electroporation chamber, and proprietary BTXPress™ Cytoporation Medium T, optimized for large volume electroporation. IQ/OQ Now available upon request.

• Scale-up
  • Transfection protocols readily scale-up from standard laboratory cuvettes to large-volume transfection in the AgilePulse™ MAX system.
• Maximal Efficiency with Cytoporation Medium
  • BTXPress™ Cytoporation Medium T used with the AgilePulse™ MAX system has been optimized for maximal efficiency with a number of cell lines, including K562, A20, HEK293 and CHO-KI.
  • Compatible with a large range of transfectants including DNA, RNA, siRNA, and olignonucleotides.
  • Can be directly diluted in complete growth medium for post-electroporation cell culture.
• Simple User Interface
  • All controls are operated with the simple touch screen on the front panel.
  • Data is quickly retrieved by USB key and can be analyzed for detailed pulse characteristics including pulse and pulse current.
• AgilePulse™ Advantage
  • Transfection efficiency and cell viability are enhanced by specialized, programmable electrical pulse waveforms, particularly important for larger polynucleotide delivery such as DNA plasmids.
  • The patented AgilePulse™ technology combines a unique sequence of short high-intensity pulses to porate cell membranes, followed by long low-intensity pulses to further drive transfectants into cells via electrophoresis, while maintaining cell viability.

Specifications

• Format: Large volume
• For Use With (Equipment): BTXpress Cytoporation Medium T or Cytoporation Medium T4, large volume electroporation chamber
• Product Line: BTX Electroporation
• Voltage: 50 to 1000 V
• Display: Touchscreen
• Depth (Metric): 40 cm
• Height (Metric): 20 cm
• Operating Temperature (English): 50°F to 104°F
• Operating Temperature (Metric): 10°C to 40°C
• Quantity: 1 Ea.
• Weight (English): 25 lb.
• Width (English): 12.6 in.
• Sterility: Non-sterile
• Interface: Touch Screen Display, Footswitch

• For Use With (Application): CRISPR Transfections, Transfect Cells Such as Bone Marrow to Produce or Replace a Missing Protein, Deliver siRNA to Suppress Gene Expression, Deliver Genes for Permanent Gene Correction, Load Cells with a Drug for Drug Delivery, Cancer Immunotherapy, Transfect Eukaryotic Cells for Protein Production in Bioreactors, Large-Scale Production of Replication-Deficient Viruses
• Frequency: 5 kHz to 1 Hz
• Product Type: Complete Electroporation System
• Description: AgilePulse MAX Large Volume Transfection System, Complete
• Depth (English): 15.7 in.
• Height (English): 7.9 in.
• Includes: AgilePulse™ MAX Generator, Large Volume Chamber Stand, Safety Stand, 5 mL Chamber (2 X), Cytoporation Medium T (500 mL) and 4 mm gap Cuvettes
• Pulse Length Range: 0.200 to 1000 ms
• Pulse Width Range: 0.050 to 10 ms
• Volume (Metric): Up to 10 mL
• Weight (Metric): 11.3 kg
• Width (Metric): 32 cm
• Connection Type: Chamber Cable
• Pulse Form: Square Wave