Gibco™ AAV-MAX Helper-Free AAV Production System Kit, without lysis buffer

The Gibco™ AAV-MAX Helper-Free AAV Production System is a scalable and high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium.

Supplier:  Gibco™ A54824

Catalog No. A54824

Description

• A scalable and high-titer platform for suspension AAV production
• Based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium
• Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer
• The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols
• Note, this version of the kit does not contain the AAV-MAX Lysis Buffer
• To purchase the kit including the AAV-MAX Lysis Buffer, please see A51217
• All components are also available for purchase separately

Features of the AAV-MAX Helper-Free AAV Production System include:

• Components work synergistically to produce high AAV titers greater than 5x10¹⁰ vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large scale transfection

Components of the AAV-MAX System Kit include:

• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL GlutaMAX™ Supplement

Specifications

• Content And Storage: • 1 vial Viral Production Cells 2.0
  • 1 L Viral Production Medium
  • 1 AAV-MAX Transfection kit (for 1 L production)
  • 100 mL Viral-Plex Complexation Buffer
  • 100 mL GlutaMAX
  
  
• Cell Type: Kidney (Embryonic)
• Product Type: AAV Production System
• For Use With (Application): AAV production
• Quantity: 1 kit

• Cell Line: Viral Production Cells 2.0
• Culture Type: Suspension Cell Culture
• Sterility: Sterile-filtered
• Product Line: AAV-MAX

Safety and Handling

ShelfLife : 12 months from date of manufacture