Typically used to modify oligos slated for RNA or RNA-protein structural studies. 4-Thiouridine, MP Biomedicals is an anti-metabolite and a photoactivable uridine analogue used as an affinity label. It is a thiol-modified ribonucleoside in which the oxyge
4-Thiouridine is an enzyme inhibitor, generally irreversible, that is similar in structure to a particular substrate for a specific enzyme. 4-Thiouridine is typically used to modify oligos slated for RNA, or RNA-protein, structural studies.
- A 4-thio-rU modified RNA pentamer was used to study the effect of this modification on codon-anticodon interaction when it is in the wobble position of tRNA.
- 4-thio-rU modified RNA oligos have been used as photoaffinity probes in the role of substrate analogs for characterizing the enzyme:substrate complex of tRNA:pseudouridine-5S synthase.
- 4-thio-rU modified oligos have been used as modules for assembling U25 small nucleolar RNAs (U25snoRNA) by ligation. These snoRNAs were used in cross-linking studies to identify which proteins assembled on them in vivo in Xenopus oocytes.
- Because the thiol group is chemically reactive, other moieties can be conjugated at the thiol group of 4-thio-rU. Such a strategy was used to introduce spin labels to 4-thio-rU-containing RNA oligos.