Cytiva 2-D Quant Kit

Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. Cytiva Life Sciences™ 2-D Quant Kit  precipitates proteins while leaving interfering substances behind.

Supplier:  Cytiva 80648356

Catalog No. 45-001-732

Includes: Precipitant, 250mL; Co-precipitant, 250mL; Copper solution, 50mL; Color reagent A, 2 x 250mL; Color reagent B, 5mL; BSA standard (2mg/mL), 5mL

Description

Designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques. Works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein.

• Works in the presence of 2% SDS, 1% DTT, 8M urea, 2M thiourea, 4% CHAPS, 2% Pharmalyte™, and 2% IPG Buffer
• Quantitatively precipitates proteins while leaving interfering substances behind
• Linear response in the range of 0 to 50μg protein, with sample volumes of 1 to 50μL
• Assay time: one hour

2-D electrophoresis, SDS-PAGE, IEF

Specifications

• For Use With (Application): 2D Electrophoresis, SDS-PAGE, IEF
• Sample Volume: 1 to 50μL
• Product Type: 2D Quant kit

• Includes: 250mL Precipitant, 250mL Co Precipitant, 50mL Copper Solution, 2 x 250mL Color reagent A, 5mL Color reagent B, 5mL BSA standard (2mg/mL)
• Content And Storage: 4° to 8°C
• Quantity: 500 Assays