Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View details.
Catalog # OPA1-10080
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:200 |
Immunocytochemistry (ICC/IF) | 1:200 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to the carboxy terminal region of mouse p38. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 0.2 mg/mL |
Purification | Affinity chromatography |
Storage buffer | PBS with 0.2% gelatin |
Contains | 0.1% sodium azide |
Storage conditions | 4° C, do not freeze |
RRID | AB_325382 |
OPA1-10080 detects p38 from human, mouse and rat samples.
OPA1-10080 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects an ~38 kDa protein representing p38 from HeLa whole cell lysates.
OPA1-10080 immunizing peptide corresponds to the carboxy terminal region of mouse p38.
The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CRK1; CSAID-binding protein; Csaids binding protein; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; Cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; MAP kinase 14; MAP kinase 2; MAP kinase MXI2; MAP kinase p38 alpha; MAPK 14; MAX-interacting protein 2; mitogen activated protein kinase 14; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; Mitogen-activated protein kinase p38 alpha; p38 alpha; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38alpha Exip; reactive kinase; SAPK2a; Stress-activated protein kinase 2a; tRNA synthetase cofactor p38
Gene Aliases: CRK1; CSBP; CSBP1; CSBP2; CSPB1; EXIP; Hog; MAPK14; MXI2; p38; p38-alpha; p38a; p38ALPHA; p38Hog; p38MAPK; PRKM14; PRKM15; RK; SAPK2A
UniProt ID: (Human) Q16539, (Mouse) P47811
Entrez Gene ID: (Human) 1432, (Rat) 81649, (Mouse) 26416
Molecular Function:
non-receptor serine/threonine protein kinase
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