Catalog # PA3-032A
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | 1:800 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1:200-1:400 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | 1:250 |
PUBLISHED APPLICATIONS | |
---|---|
Immunohistochemistry (IHC) | See 7 publications below |
Immunocytochemistry (ICC/IF) | See 2 publications below |
Immunoprecipitation (IP) | See 2 publications below |
Western Blot (WB) | See 6 publications below |
Immunohistochemistry (Paraffin) (IHC (P)) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Immunohistochemistry (Frozen) (IHC (F)) | See 1 publications below |
Species reactivity | Bovine, Human, Mouse, Rabbit, Rat |
Published species | Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat bNOS. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage buffer | whole serum |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2236017 |
PA3-032A detects brain nitric oxide synthase (bNOS) from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
PA3-032A has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects an ~155 kDa protein representing bNOS from rat brain extract. Immunohistochemical staining of bNOS in rat brain with PA3-032A results in staining of the anterior pituitary.
The PA3-032A immunogen is a synthetic peptide corresponding to residues T(724) K R R A I G F K K L A E A V K(739) C of rat nNOS.
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Gene Aliases: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1
UniProt ID: (Human) P29475, (Rabbit) O19132, (Rat) P29476, (Mouse) Q9Z0J4
Entrez Gene ID: (Human) 4842, (Rabbit) 100009243, (Rat) 24598, (Mouse) 18125
Molecular Function:
oxidoreductase
protein modifying enzyme
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