Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.
TESTED APPLICATIONS | DILUTION |
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Immunohistochemistry (IHC) | 10-50 µg/mL |
Western Blot (WB) | 10-50 µg/mL |
PUBLISHED APPLICATIONS | |
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Immunohistochemistry (IHC) | See 1 publications below |
Published species | Mouse |
Host / Isotype | Goat / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptides from the c-terminal region of human NOS1 conjugated to blue carrier protein was used as the antigen. |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | 1 mg/mL |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
RRID | AB_10709561 |
Glycerol (1:1) may be added for additional stability.
Reconstitute with 500 µL of distilled water.
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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