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eNOS Polyclonal Antibody

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.

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Catalog # PA1-037

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eNOS Antibody in Immunohistochemistry (IHC) — Immunolocalization staining of eNOS in human lung tissue using Product # PA1-037.

eNOS Antibody — The specificity of anti-eNOS polyclonal antibody (Product # PA1-037) was demonstrated using naturally occurring variable expression to confirm specificity. Immunohistochemistry analysis of human tissue sections using this antibody showed eNOS is strongly stained in human spleen tissue and no staining in human tonsil tissue which does not express eNOS. {RE}

eNOS Antibody in Western Blot (WB) — Figure 2 BPF pre- and perinatal effect on nitrosative stress markers in the liver of female and male PND6 offspring. ( a ) mRNA levels of eNOS, iNOS and HO-1d in female offspring; ( b ) mRNA levels of eNOS, iNOS and HO-1d in male offspring; ( c ) protein expression of eNOS, iNOS and HO-1d in female offspring; ( d ) protein expression of eNOS, iNOS and HO-1d in male offspring; and ( e ) representative eNOS, iNOS and HO-1d protein blots measured by Western blotting in both sexes. Data represent mean +- SD. For mRNA analysis, n = 12 female PND6 pups and n = 12 male PND6 pups for each experimental group with three replicates for each sample, control (C), low-dose BPF (LBPF) and high-dose BPF (HBPF), were evaluated, and for protein analysis, n = 5 female and n = 5 male per experimental group. Statistical significance was determined by one-way ANOVA. * p < 0.05; ** p < 0.01 compared to control group. p < 0.05; < 0.01, LBPF vs. HBPF.

eNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — 3 FIGURE Immunohistochemical (IHC) staining for detection of NO synthases and antioxidant enzymes in patient pericardial resistance arteries illustrated by typical examples and results of semi-quantitative analyses summarized by violin plots ( N = 28). (a and b) Endothelial NO synthase (eNOS) in a pericardial resistance artery and an atrial appendage, respectively. (c) Inducible NO synthase (iNOS), (d) neuronal NO synthase (nNOS), (e) catalase (CAT) and (f) superoxide dismutase 1 (SOD1) in pericardial resistance arteries. EC, endothelium; SMC, medial smooth muscle; ADV, tunica adventitia. Scale bar 50 mum

eNOS Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P)) — Published figure using eNOS polyclonal antibody (Product # PA1-037) in Western Blot and Immunohistochemistry (Paraffin)

eNOS Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P)) — Published figure using eNOS polyclonal antibody (Product # PA1-037) in Immunohistochemistry and Immunohistochemistry (Paraffin)

eNOS Antibody in ChIP Assay (ChIP) — Published figure using eNOS polyclonal antibody (Product # PA1-037) in ChIP Assay

Product Details

TESTED APPLICATIONS	DILUTION
Immunohistochemistry (Frozen) (IHC (F))	1:100
Immunohistochemistry (Paraffin) (IHC (P))	1:100
Western Blot (WB)	1:1,000

PUBLISHED APPLICATIONS
Western Blot (WB)	See 30 publications below
Immunohistochemistry (Paraffin) (IHC (P))	See 5 publications below
Immunohistochemistry (IHC)	See 33 publications below
Immunoprecipitation (IP)	See 1 publications below
ChIP assay (ChIP)	See 1 publications below
Immunocytochemistry (ICC/IF)	See 3 publications below
Immunomicroscopy (IM)	See 1 publications below

Species reactivity	Bovine, Dog, Human, Mouse, Pig, Rat
Published species	Amphibian, Bovine, Cat, Dog, Fruit fly, Human, Mouse, Rabbit, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_325774

Product Specific Information

PA1-037 detects endothelial nitric oxide synthase (eNOS) from bovine, canine, human, mouse, porcine and rat tissues as well as recombinant protein. This antibody does not detect human iNOS or rat nNOS.

PA1-037 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. By Western blot PA1-037 also detects eNOS in rat lung, brain, and canine cardiac microsomes. Immunohistochemical staining of eNOS in human vascular endothelial cells with PA1-037 results in prominent staining of the epithelial cells.

The PA1-037 immunogen is a synthetic peptide corresponding to residues S(1179) L Q E R Q L R G A V P W A F D(1194) of human eNOS.

Background/Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (53)

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Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; Endothelial NOS; eNOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

Gene Aliases: 2310065A03Rik; cNOS; EC-NOS; ECNOS; eNOS; NOS; Nos-3; NOS3; NOSIII

UniProt ID: (Pig) Q28969, (Human) P29474, (Mouse) P70313, (Rat) O88672

Entrez Gene ID: (Bovine) 287024, (Pig) 397557, (Human) 4846, (Dog) 403784, (Mouse) 18127, (Rat) 24600

Molecular Function: Blue Arrow Icon oxidoreductase protein modifying enzyme

Function(s)

arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity oxidation-reduction process cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion positive regulation of blood vessel diameter angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin nitric oxide mediated signal transduction regulation of blood pressure negative regulation of cell proliferation response to heat negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development lipopolysaccharide-mediated signaling pathway response to fluid shear stress negative regulation of potassium ion transport endothelial cell migration cell redox homeostasis positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation regulation of blood vessel size regulation of nitric-oxide synthase activity negative regulation of hydrolase activity negative regulation of calcium ion transport signal transduction response to estradiol positive regulation of apoptotic process negative regulation of smooth muscle cell proliferation cellular response to mechanical stimulus cellular response to transforming growth factor beta stimulus response to hypoxia female pregnancy aging response to mechanical stimulus response to fructose response to metal ion response to lead ion response to organic cyclic compound response to activity response to lipopolysaccharide response to cytokine cellular response to heat response to statin response to drug response to amino acid response to peptide hormone response to ethanol response to axon injury response to corticosterone response to hyperoxia response to lipoprotein particle retina development in camera-type eye bone development cellular response to magnesium ion cellular response to cAMP cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to organic cyclic compound response to candesartan response to peptide response to ketone response to salt

Process(es)

nitric-oxide synthase activity
calmodulin binding
FMN binding
heme binding
metal ion binding
flavin adenine dinucleotide binding
NADP binding
actin monomer binding
iron ion binding
protein binding
tetrahydrobiopterin binding
arginine binding
cadmium ion binding
scaffold protein binding
actin binding
NADPH-hemoprotein reductase activity
beta-catenin binding
oxidoreductase activity
cadherin binding
nitric-oxide synthase binding
Hsp90 protein binding

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eNOS Polyclonal Antibody

Product Details

Product Specific Information

Background/Target Information

References (53)

Have you cited this antibody in a publication? so we can reference it in this datasheet.

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

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eNOS Polyclonal Antibody

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Product Details

Product Specific Information

Background/Target Information

References (53)

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

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