Catalog # HYB 094-06-02
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | 1:6,500 |
Western Blot (WB) | 1:2,000 |
PUBLISHED APPLICATIONS | |
---|---|
ELISA (ELISA) | See 1 publications below |
Published species | Not Applicable |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Clone | 6C8 |
Immunogen | Ovalbumin isolated from chicken egg white, denatured by heat and formaldehyde. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.07 mg/mL |
Storage conditions | 4° C, store in dark |
RRID | AB_1072960 |
HYB 094-06-02 HYB 094-06 is specific for denatured chicken ovalbumin (OA). Cross-reactivity to egg albumines from other species has not been tested.
HYB 094-06-02 has been successfully used in ELISA and Western blotting procedures. HYB 094-06-02 reacts strongly with OA in denatured and modified forms (native OA surface-adsorbed, heat-denatured or reduced). Strong reaction is seen in ELISA with OA directly coated onto the microtiter well. In dotblotting, HYB 094-06 reacts with both native and denatured OA adsorbed onto nitrocellulose. In Western blotting after SDS-PAGE, HYB 094-06-02 reacts with OA in reduced as well as unreduced forms.
The HYB 094-06-02 immunogen is OA isolated from chicken egg white, heat-denatured.
Epitope specificity differs from that of HYB 094-05-02 and HYB 094-07-02, as determined by inhibition ELISA.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Ovalbumin (OA), formaldehyde treated and heat denatured by incubation for 1 h at 105°C. OA is isolated from chicken egg white in which it makes up about 60% of solids. Native ovalbumin is a globular monomer of molecular mass ~45 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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