Invitrogen
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-91399
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Western Blot (WB) | 1:5,000-1:10,000 |
Immunocytochemistry (ICC/IF) | 1:1,000-1:2,000 |
PUBLISHED APPLICATIONS | |
---|---|
Flow Cytometry (Flow) | See 1 publications below |
Western Blot (WB) | See 33 publications below |
Immunohistochemistry (IHC) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 4 publications below |
Species reactivity | Bovine, Dog, Chicken, Cat, Guinea pig, Human, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus |
Published species | Bovine, Human, Mouse, Rat, Sheep, Zebrafish |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | AC-15 |
Immunogen | Slightly modified beta cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 3 mg/mL |
Storage buffer | Ascites |
Contains | 15mM sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2273656 |
Positive control of cultured human or chicken fibroblasts suggested.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: actin, beta; actin, beta, cytoplasmic; Actin, cytoplasmic 1; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic 1; Cytoplasmic beta-actin; cytoskeletal beta actin; cytoskeletal protein; gamma non-muscle actin; PS1TP5-binding protein 1
Gene Aliases: ACT-5; ACTB; actin; Actx; Bact; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1; XELAEV_18045052mg
UniProt ID: (Sheep) P60713, (Rabbit) P29751, (Bovine) P60712, (Human) Q96B34, (Pig) Q6QAQ1, (Chicken) P60706, (Dog) O18840, (Guinea pig) Q71FK5, (Rat) P60711, (Mouse) P60710, (Xenopus) O93400
Entrez Gene ID: (Sheep) 443052, (Rabbit) 100009272, (Bovine) 280979, (Human) 60, (Pig) 414396, (Chicken) 396526, (Dog) 403580, (Guinea pig) 100135470, (Rat) 81822, (Mouse) 11461, (Xenopus) 398459
Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein
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