TESTED APPLICATIONS | DILUTION |
---|---|
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | Assay-dependent |
Immunocytochemistry (ICC/IF) | 1:500 |
PUBLISHED APPLICATIONS | |
---|---|
Gel Shift (GS) | See 1 publications below |
Published species | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Full length native alpha actinin purified from chicken gizzard. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_1956052 |
Recommended positive controls: chicken fibroblast, chicken gizzard.
Expected to cross-react with a wide range of tther species due to sequence homology.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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