Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.
Catalog # CSI 003-02-02
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
Western Blot (WB) | 1:100-1:1,000 |
Immunocytochemistry (ICC/IF) | 1:200 |
Species reactivity | Human |
Host / Isotype | Mouse / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Clone | HV2 |
Immunogen | Human vitronectin purified from plasma by heparin-affinity chromatography. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.0 mg/mL |
Purification | Protein A |
Storage buffer | PBS, pH 7.4 |
Contains | 15mM sodium azide |
Storage conditions | 4° C, store in dark |
RRID | AB_1088278 |
CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse).
CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands.
The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: complement S-protein; epibolin; S-protein; serum spreading factor; Serum-spreading factor; somatomedin B; V75; Vitronectin; VN
Gene Aliases: V75; VN; VNT; VTN
UniProt ID: (Human) Q9BSH7
Entrez Gene ID: (Human) 7448
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