Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-768
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Western Blot (WB) | 2 µg/mL |
Immunocytochemistry (ICC/IF) | 2 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 3 publications below |
Immunocytochemistry (ICC/IF) | See 4 publications below |
Immunohistochemistry (IHC) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant rat VAMP-4 protein. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2212790 |
PA1-768 detects VAMP-4 from human and mouse samples.
PA1-768 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract. Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a staining pattern consistent with VAMP-4 staining.
Immunofluorescence staining with this antibody requires saponin permeabilization.
The PA1-768 antigen is recombinant rat VAMP-4.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, VAMP levels of all isoforms appear to be significantly lowered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: vamp; VAMP-4; vesicle associated membrane protein 4; Vesicle-associated membrane protein 4
Gene Aliases: D1Ertd147e; VAMP-4; VAMP24; VAMP4
UniProt ID: (Human) O75379, (Mouse) O70480
Entrez Gene ID: (Human) 8674, (Mouse) 53330, (Rat) 364033
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.