Invitrogen
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (IHC) | 1:300-1:1,000 |
Western Blot (WB) | 1:300-1:1,000 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | A synthetic peptide from human ULK1 conjugated to blue carrier protein has been used as the antigen. |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | Conc. Not Determined |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
RRID | AB_1091092 |
Reconstitute with 150 µL of distilled water.
The peptide is homologous in rat and mouse.
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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