TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (IHC) | 1:300-1:2,000 |
Western Blot (WB) | 1:300-1:2,000 |
PUBLISHED APPLICATIONS | |
---|---|
Neutralization (Neu) | See 1 publications below |
Published species | Rat |
Host / Isotype | Sheep / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | A synthetic peptide from aa region 400-450 of the extracellular domain of mouse TrkB conjugated to an immunogenic carrier protein was used as the antigen. |
Conjugate | Unconjugated |
Form | Lyophilized |
Concentration | Conc. Not Determined |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
RRID | AB_10710003 |
Glycerol (1:1) may be added for additional stability.
Reconstitute in 100 µl of sterile water.
NTRK2 (TRKB) belongs to the neurotrophic factor family of related polypeptides central to the development and maintenance of the mammalian nervous system. NTRK2 is the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses. TrkB is involved in the development and maintenance of the nervous system. Moreover, Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. TrkB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for the TrkB gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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