Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
TESTED APPLICATIONS | DILUTION |
---|---|
Immunocytochemistry (ICC/IF) | 1-2 µg/mL |
PUBLISHED APPLICATIONS | |
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Immunocytochemistry (ICC/IF) | See 10 publications below |
Western Blot (WB) | See 6 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Published species | Human, Mouse, Rhesus monkey |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | 236-15375 |
Immunogen | human Transferrin Receptor |
Conjugate | Unconjugated |
Form | Lyophilized |
Storage conditions | -20°C |
RRID | AB_2534136 |
The anti-human transferrin receptor antibody should be useful for the localization of transferrin receptors by immunofluorescence techniques. This antibody recognizes the human transferrin receptor and does not block binding of transferrin to the receptor.
Although fluorescent transferrin conjugates have been widely used to study transferrin receptor-mediated endocytosis, antibodies to the transferrin receptor have proven to be useful for localizing transferrin receptors, studying endocytic recycling pathways and performing enzyme-linked immunosorbent assays (ELISAs).
Upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2 mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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