Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA3-926
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
Immunohistochemistry (Paraffin) (IHC (P)) | 1/100 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:1,000 |
Immunocytochemistry (ICC/IF) | 1:200 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 66 publications below |
Immunohistochemistry (IHC) | See 6 publications below |
Immunocytochemistry (ICC/IF) | See 12 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Species reactivity | Dog, Guinea pig, Human, Mouse, Rabbit, Rat |
Published species | Bovine, Dog, Guinea pig, Human, Marsupial, Mouse, Pig, Rabbit, Rat, Rodent |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Clone | C2C12 |
Immunogen | Purified canine cardiac sodium/calcium exchanger. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Affinity chromatography - MBP |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2191111 |
MA3-926 detects sodium/calcium exchanger from human, canine, rabbit, mouse, guinea pig and rat kidney and cardiac tissues.
MA3-926 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 120 kDa protein representing the sodium/calcium exchanger from guinea pig cardiac extract. The bands seen at 70 kDa and 160 kDa represent a proteolytic fragment and non-reduced exchanger respectively. MA3-926 is not recommended for Western blot procedures of rat tissues. Immunohistochemical staining of the sodium/calcium exchanger in rat heart with MA3-926 results in staining of the plasma membrane and intense staining of cardiac T-tubular membrane.
The MA3-926 antigen is purified canine cardiac sodium/calcium exchanger. This antibody recognizes an epitope between amino acids 371-525, which is on the intracellular side of the plasma membrane.
Ca2+ plays a critical role in intracellular signaling and thus regulation of calcium balance is critical for the maintenance of this signaling function. The plasma membrane Na+/Ca2+ (NaCX) exchangers mediate Na+-dependent Ca2+ efflux in a wide variety of cell types. NaCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In mammals, at least 5 distinct genes code for the exchangers: Three NaCX (NaCX-1, NaCX-2, and NaCX-3), and two in the NaCKX family (NaCKX-1 and NaCKX-2). NaCX-1 is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling (Matsuoka et al., 1993). NACX-1 may also be a target for protection of the heart and brain from ischemia and reperfusion injury (Hagihara et al., 2005; Bano et al., 2005; Choi, 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cardiac sarcolemmal sodium-calcium exchanger; isoform NCX1.13; isoform NCX1.3; isoform NCX1.9; Na(+)/Ca(2+)-exchange protein 1; Na+/C2+ exchanger; Na+/Ca++ exchanger; Na+/Ca2+ exchanger; Na-Ca exchanger; Na/Ca exchanger; NKX; sodium-calcium exchanger; sodium/calcium exchanger; Sodium/calcium exchanger 1; sodium/calcium exchanger isoform NaCa13; sodium/calcium exchanger isoform NaCa3; sodium/calcium exchanger isoform NaCa7; sodium/calcium exchanger isoform NaCa9; solute carrier family 8 (sodium/calcium exchanger), member 1; Solute carrier family 8 member 1; solute carrier family 8, member 1
Gene Aliases: AI852629; AV344025; CNC; D930008O12Rik; NACA; Ncx; NCX1; SLC8A1
UniProt ID: (Dog) P23685, (Human) P32418, (Mouse) P70414, (Rat) Q01728, (Guinea pig) P48766
Entrez Gene ID: (Dog) 475738, (Rabbit) 100328570, (Human) 6546, (Mouse) 20541, (Rat) 29715, (Guinea pig) 100135620
Molecular Function:
secondary carrier transporter
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