Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-31072
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (IHC) | 1 µg/mL |
Immunoprecipitation (IP) | 10 µg/mL |
Western Blot (WB) | 1:1,000-1:5,000 |
Immunocytochemistry (ICC/IF) | 1:100 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 2 publications below |
Species reactivity | Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus |
Published species | Bovine, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Full length protein (Human). |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Affinity chromatography |
Storage buffer | 0.05M sodium phosphate, pH 7.2, with 0.1% BSA, 50% glycerol |
Contains | 0.1% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_2286296 |
Rat brain tissue extract was used as a positive control.
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: indophenoloxidase B; IPO B; manganese SOD; manganese superoxide dismutase; manganese-containing superoxide dismutase; mangano-superoxide dismutase; manganous superoxide dismutase; mitochondrial superoxide dismutase 2; Mn superoxide dismutase; Superoxide dimutase 2, mitochondrial; superoxide dismutase; superoxide dismutase (Mn type); superoxide dismutase 2, mitochondrial; superoxide dismutase 2, mitochondrial L homeolog; Superoxide dismutase [Mn], mitochondrial
Gene Aliases: I79_015272; IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; Sod-2; SOD2; sodMt
UniProt ID: (Bovine) P41976, (Human) Q5TCM1, (Rat) P07895, (Mouse) P09671
Entrez Gene ID: (Bovine) 281496, (Human) 6648, (Rabbit) 103349944, (Dog) 476258, (Sheep) 780457, (Pig) 100154319, (Rat) 24787, (Chinese hamster) 100760320, (Mouse) 20656, (Xenopus) 399216
Molecular Function:
oxidoreductase
metabolite interconversion enzyme
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.