Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA3-910
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Flow Cytometry (Flow) | 1 µg / 10^6 cells |
Immunohistochemistry (Frozen) (IHC (F)) | 1:500 |
Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
Western Blot (WB) | 1:500-1:5,000 |
Immunocytochemistry (ICC/IF) | 5 µg/mL |
PUBLISHED APPLICATIONS | |
---|---|
Immunoprecipitation (IP) | See 4 publications below |
Western Blot (WB) | See 85 publications below |
Immunohistochemistry (IHC) | See 18 publications below |
Immunocytochemistry (ICC/IF) | See 31 publications below |
ChIP assay (ChIP) | See 1 publications below |
Miscellaneous PubMed (Misc) | See 1 publications below |
ELISA (ELISA) | See 1 publications below |
Immunohistochemistry (Frozen) (IHC (F)) | See 1 publications below |
Immunomicroscopy (IM) | See 1 publications below |
Species reactivity | Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat |
Published species | Amphibian, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Sheep, Virus, Zebrafish |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Clone | IID8 |
Immunogen | Purified canine cardiac sarcoplasmic reticulum. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Affinity chromatography |
Storage buffer | PBS |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2227681 |
MA3-910 detects sarcoplasmic or endoplasmic reticulum calcium 2 (SERCA2) ATPase from bovine, canine, human, pig, rabbit and rat tissues. This antibody does not detect frog SERCA2 ATPase (J. Biol. Chem., Feb 2001; 276: 3911 - 3919). MA3-910 detects SERCA2 ATPase and does not discriminate between the two isoforms.
MA3-910 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle with MA3-910 results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization.
MA3-910 is not recommended for Western blot detection of rat SERCA2.
The MA3-910 antigen is purified canine cardiac sarcoplasmic reticulum.
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; ATPase, Ca++ transporting, slow twitch 2; Ca(2+)-transport ATPase class 3; calcium ATPase; Calcium pump 2; calcium-ATPase (EC 3.6.1.3); Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarco(endo)plasmic reticulum Ca(2+)-dependent ATPase 2; sarco/endoplasmic reticulum Ca2+-ATPase 2; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; sarcoplasmic/endoplasmic-reticulum Ca(2+) pump gene 2; SERCA; SERCA ATPase; SERCA2; SR Ca(2+)-ATPase 2; unnamed protein product
Gene Aliases: 9530097L16Rik; ATP2; ATP2A2; ATP2B; D5Wsu150e; DAR; DD; mKIAA4195; SERCA2; SERCA2A; SERCA2B; SercaII
UniProt ID: (Dog) O46674, (Rabbit) P20647, (Human) P16615, (Pig) P11607, (Mouse) O55143, (Rat) P11507
Entrez Gene ID: (Dog) 403878, (Bovine) 540568, (Rabbit) 100038308, (Human) 488, (Pig) 396875, (Mouse) 11938, (Rat) 29693
Molecular Function:
primary active transporter
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