Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-741
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:500-1:5,000 |
Immunocytochemistry (ICC/IF) | 1:200 - 1:250 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 16 publications below |
Immunohistochemistry (IHC) | See 5 publications below |
Immunocytochemistry (ICC/IF) | See 6 publications below |
Immunoprecipitation (IP) | See 5 publications below |
Immunohistochemistry - Free Floating (IHC (Free)) | See 1 publications below |
Species reactivity | Human, Mouse, Rat |
Published species | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic peptide corresponding to residues V(115) L P S E R I S P Q V P N E V L G P E(133) of rat SAP97. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | Conc. Not Determined |
Purification | Ammonium sulfate precipitation |
Storage buffer | PBS |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2092020 |
PA1-741 detects Synapse-Associated Protein 97 (SAP97) from human, rat and mouse tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.
PA1-741 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain extract. Immunohistochemical staining of SAP97 in rat hippocampus with PA1-741 results in intense neuronal cell body and dendrite staining.
The PA1-741 immunizing peptide corresponds to amino acids 115-133 from rat SAP97. This sequence is 84% and 74% conserved in mouse and human SAP97, respectively.
PA1-741 can be used with blocking peptide PEP-055.
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: discs large homolog 1; discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; discs, large homolog 1 (Drosophila) transcript variant 6-v1; discs, large homolog 1 (Drosophila) transcript variant 6-v2; Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); E-dlg/SAP97; Embryo-dlg/synapse-associated protein 97; hDlg; presynaptic protein SAP97; SAP-97; Synapse-associated protein 97
Gene Aliases: B130052P05Rik; dJ1061C18.1.1; DLG1; DLGH1; E-dlg/SAP97; hdlg; mKIAA4187; SAP-97; SAP97
UniProt ID: (Human) Q12959, (Rat) Q62696, (Mouse) Q811D0
Entrez Gene ID: (Human) 1739, (Rat) 25252, (Mouse) 13383
Molecular Function: scaffold/adaptor protein
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