Novus Biologicals

Manufacturer Code:NBP266801

RNA Polymerase II/POLR2A (ac Ser5) Rabbit anti-Human, Mouse, Rat, Clone: JM51-21, Novus Biologicals™

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Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A [ac Ser5] Antibody (JM51-21) [NBP2-66801] — Staining Phospho-POLR2A (S5) in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [ac Ser5] Antibody (JM51-21) [NBP2-66801] — Analysis of paraffin-embedded human kidney tissue using anti- Phospho-POLR2A (S5) antibody. Counter stained with hematoxylin.

Western Blot: RNA Polymerase II/POLR2A [ac Ser5] Antibody (JM51-21) [NBP2-66801] — Analysis of Phospho-POLR2A (S5) on different cells lysates using anti- Phospho-POLR2A (S5) antibody at 1/500 dilution. Positive control: Line 1: Hela Line 2: MCF-7

Flow Cytometry: RNA Polymerase II/POLR2A [ac Ser5] Antibody (JM51-21) [NBP2-66801] — Analysis of Hela cells with Phospho-POLR2A (S5) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibod

Product Details

TESTED APPLICATIONS	DILUTION
ChIP assay (ChIP)	Assay dependent
Flow Cytometry (Flow)	Assay dependent
Immunocytochemistry (ICC)	Assay dependent
Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (IHC)	Assay dependent
Immunoprecipitation (IP)	Assay dependent
Western Blot (WB)	Assay dependent

Species reactivity	Many
Host / Isotype	Rabbit
Class	Monoclonal
Type	Antibody
Clone	JM51-21
Immunogen	Synthetic phospho-peptide corresponding to residues surrounding Ser5 of human POLR2A
Conjugate	Unconjugated
Form	Liquid
Purification	purified
Storage buffer	PBS, pH7.4
Contains	15mM sodium azide
Storage conditions	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product Specific Information

Rabbit Monoclonal Antibody

Bioinformatics

Protein Aliases: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kdsubunit DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1 DNA-directed RNA polymerase III largest subunit EC 2.7.7.48 EC 2.7.7.6 hRPB220 hsRPB1 MGC75453 POLR2 POLRA polymerase (RNA) II (DNA directed) polypeptide A (220kD) polymerase (RNA) II (DNA directed) polypeptide A 220kDa RNA Pol 2 RNA Polymerase 2 RNA polymerase II subunit B1 RNA-directed RNA polymerase II subunit RPB1 RNAPII RPB1 RPBh1 RpIILS RPO2 RPOL2; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1

Gene Aliases: hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

UniProt ID: (Human) P24928

Entrez Gene ID: (Human) 5430

Molecular Function: Blue Arrow Icon DNA-directed RNA polymerase RNA binding protein nucleic acid binding nucleotidyltransferase transferase

Function(s)

mRNA splicing, via spliceosome transcription, RNA-templated transcription-coupled nucleotide-excision repair DNA-templated transcription, termination regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA positive regulation of RNA splicing somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription

Process(es)

RNA polymerase II activity
DNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
ubiquitin protein ligase binding
poly(A) RNA binding
metal ion binding

Suggested Secondary Antibodies

Anti-Rabbit Secondary Antibodies (730)

Alexa Fluor® Anti-Rabbit Secondary Antibodies (91)

HRP Anti-Rabbit Secondary Antibodies (100)

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If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*

RNA Polymerase II/POLR2A (ac Ser5) Rabbit anti-Human, Mouse, Rat, Clone: JM51-21, Novus Biologicals™

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RNA Polymerase II/POLR2A (ac Ser5) Rabbit anti-Human, Mouse, Rat, Clone: JM51-21, Novus Biologicals™

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Product Details

Product Specific Information

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Your items have has been added!