Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View details.
Catalog # MA1-23271
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:100-1:1,000 |
Immunoprecipitation (IP) | Assay-dependent |
Western Blot (WB) | 1:500-1:3,000 |
in situ PLA (PLA) | Assay-dependent |
Immunocytochemistry (ICC/IF) | 1:100-1:1,000 |
PUBLISHED APPLICATIONS | |
---|---|
Immunocytochemistry (ICC/IF) | See 2 publications below |
Species reactivity | Chicken, Human, Mouse, Rat |
Published species | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Clone | 14B4 |
Immunogen | Full length Rad51 protein (amino acids 1-338) expressed in E. coli. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1.0 mg/mL |
Purification | Affinity chromatography |
Storage buffer | PBS, pH 7 |
Contains | no preservative |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_560832 |
A suggested positive control for this product is T24.
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603
UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297
Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361, (Rat) 499870
Molecular Function:
DNA metabolism protein
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