Catalog # PA5-50101
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:50-1:100 |
Western Blot (WB) | 1:500-1:3,000 |
Immunocytochemistry (ICC/IF) | 1:50 |
PUBLISHED APPLICATIONS | |
---|---|
Immunoprecipitation (IP) | See 1 publications below |
Species reactivity | Human, Mouse |
Published species | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthesized peptide derived from internal of human PC. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 50% glycerol |
Contains | 0.02% sodium azide |
Storage conditions | -20°C |
RRID | AB_2635554 |
The antibody detects endogenous levels of total Pyruvate Carboxylase protein.
Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: EC 6.4.1.1; PCB; PYC; Pyruvate carboxylase, mitochondrial; pyruvate decarboxylase; Pyruvic carboxylase
Gene Aliases: PC; PCB
UniProt ID: (Human) P11498
Entrez Gene ID: (Human) 5091, (Mouse) 18563
Molecular Function:
ligase
metabolite interconversion enzyme
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