Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View details.
Catalog # PA1-032
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1:500-1:5,000 |
Immunocytochemistry (ICC/IF) | 1:10-1:100 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 9 publications below |
Immunohistochemistry (IHC) | See 3 publications below |
Immunoprecipitation (IP) | See 1 publications below |
Immunocytochemistry (ICC/IF) | See 1 publications below |
Immunomicroscopy (IM) | See 1 publications below |
Species reactivity | Human, Rat |
Published species | Human, Mouse, Rabbit, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic phosphopeptide corresponding to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS with 1mg/mL BSA |
Contains | 0.05% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_325020 |
PA1-032 detects phosphorylated nNOS (Ser 1417) from rat and human samples.
PA1-032 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~140 kDa protein representing recombinant rat phospho-nNOS (Ser 1417).
The PA1-032 immunogen is a synthetic phosphopeptide to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, frog, and mouse protein. This peptide (Cat. # PEP-188) is available for use in neutralization and control experiments.
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
Gene Aliases: Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NOS; NOS1
UniProt ID: (Human) P29475, (Rat) P29476
Entrez Gene ID: (Human) 4842, (Rat) 24598
Molecular Function: oxidoreductase
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