Catalog # PA1-4645
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1:1,000 |
PUBLISHED APPLICATIONS | |
---|---|
Western Blot (WB) | See 1 publications below |
Species reactivity | Human, Rat |
Published species | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of rat TPH1 conjugated to KLH |
Conjugate | Unconjugated |
Form | Liquid |
Purification | Affinity chromatography |
Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
Contains | no preservative |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2207558 |
This antibody is predicted to react with bovine, canine, chicken, mouse and zebrafish based on 100% sequence homology.
This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser260 in Western blots of human dorsal Raphe nucleus.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase)
Gene Aliases: TPH; TPH1; TPRH; TRPH
UniProt ID: (Human) P17752, (Rat) P09810
Entrez Gene ID: (Human) 7166, (Rat) 24848
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