Invitrogen
Catalog # PA1-28462
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Immunohistochemistry (Paraffin) (IHC (P)) | 1:50-1:100 |
Western Blot (WB) | 1:500-1:1,000 |
Species reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic phosphopeptide derived from the region of human PKC delta that contains serine 645. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 50% glycerol |
Contains | 0.02% sodium azide |
Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID | AB_1959685 |
Recommended positive controls: U937 cells.
It detects a band approximately 77kDa.
The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 1-May; kinase nPKC-delta; KPCD; nPKC-delta; Protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta; Tyrosine-protein kinase PRKCD
Gene Aliases: ALPS3; CVID9; MAY1; nPKC-delta; PKCD; PRKCD
UniProt ID: (Human) Q05655
Entrez Gene ID: (Human) 5580
Molecular Function:
non-receptor serine/threonine protein kinase
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