Invitrogen
Catalog # PA1-4606
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
Western Blot (WB) | 1:1,000 |
Species reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MAP2K1/2 conjugated to KLH |
Conjugate | Unconjugated |
Form | Liquid |
Purification | Affinity chromatography |
Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
Contains | no preservative |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_560046 |
This antibody is predicted to react with bovine, canine, chicken, non-human primate, rat, Xenopus and zebrafish based on 100% sequence homology.
In Western blot, this antibody detects an ~45 kDa protein representing the Phospho-MEK1/2 (Ser218, 222) protein.
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 2; EC 2.7.12.2; ERK activator kinase 1; ERK activator kinase 2; kinase MEK1; MAP kinase kinase 1; MAP kinase kinase 2; MAP kinase/Erk kinase; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK-1; MEK-2; mitogen activated protein kinase kinase 1; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MP2K1; protein kinase, mitogen activated, kinase 1, p45; protein kinase, mitogen activated, kinase 2, p45; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Gene Aliases: AA589381; CFC3; CFC4; MAP2K1; MAP2K2; MAPKK1; MAPKK2; MEK1; MEK2; MEKK1; MK2; MKK1; MKK2; PRKMK1; PRKMK2
UniProt ID: (Human) Q02750, (Human) P36507, (Mouse) P31938, (Mouse) Q63932
Entrez Gene ID: (Human) 5604, (Human) 5605, (Mouse) 26395, (Mouse) 26396
Molecular Function:
non-receptor serine/threonine protein kinase
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