Invitrogen
Catalog # PA5-40280
Quantity:
TESTED APPLICATIONS | DILUTION |
---|---|
ELISA (ELISA) | 1:10,000 |
Immunohistochemistry (IHC) | 1:100-1:300 |
Western Blot (WB) | 1:500-1:2,000 |
Immunocytochemistry (ICC/IF) | 1:200-1:1,000 |
Species reactivity | Human, Mouse, Non-human primate, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synthesized peptide derived from human IRS-1 around the phosphorylation site of S323. |
Conjugate | Unconjugated |
Form | Liquid |
Concentration | 1 mg/mL |
Purification | Antigen affinity chromatography |
Storage buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
Contains | 0.02% sodium azide |
Storage conditions | -20° C, Avoid Freeze/Thaw Cycles |
RRID | AB_2608896 |
This antibody is predicted to react with mouse, rat, and monkey based on sequence homology.
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM
UniProt ID: (Human) P35568, (Rat) P35570, (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Rat) 25467, (Mouse) 16367
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